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  • Short-Term Sensitization of Colon Mechanoreceptors Is Associated With . . .
    Mice with zymosan-induced behavioral hypersensitivity 4 or 7 days after intracolonic treatment were killed via CO 2 inhalation for electrophysiologic characterization of pelvic nerve colon mechanoreceptors
  • Short-Term Sensitization of Colon Mechanoreceptors Is . . . - ScienceDirect
    Methods: Visceral nociceptive behavior was measured as the visceromotor response (VMR) to colorectal distention (CRD) before and after intracolonic treatment with zymosan or saline Colon pathology was assessed in parallel experiments by quantifying myeloperoxidase activity, intralumenal pH, and tissue histology
  • Long-term sensitization of mechanosensitive and -insensitive afferents . . .
    Accordingly, we used a model of persistent colorectal hypersensitivity induced by intracolonic zymosan and characterized mechanosensitive and -insensitive PN afferent endings, positing that MIA proportions would be significantly reduced
  • Piezo2 channels expressed by colon-innervating TRPV1-lineage neurons . . .
    Xie et al show that Piezo2 channels in TRPV1-expressing visceral nociceptors play a major role in visceral pain in mice Ablation of TRPV1-expressing nociceptors or selective knockout of Piezo2 from TRPV1 lineage neurons reduces colorectal pain under physiological conditions and in mouse models of zymosan-induced IBS and partial colon obstruction
  • (111) Role of P2X receptors in colon hypersensitivity in the mouse
    Visceral nociceptive behavior was measured as the visceromotor response (VMR) to colon distention before and after intracolonic treatment with zymosan (which reproduces major features of IBS – persistent hypersensitivity without colon inflammation) or saline
  • Short-term sensitization of colon mechanoreceptors is associated with . . .
    Visceral nociceptive behavior was measured as the visceromotor response (VMR) to colorectal distention (CRD) before and after intracolonic treatment with zymosan or saline Colon pathology was assessed in parallel experiments by quantifying myeloperoxidase activity, intralumenal pH, and tissue histology
  • SPARC: Sex difference in colorectal afferent sensitization in zymosan . . .
    In contrast, female mice showed no difference in the proportion of colorectal neurons between saline- and zymosan-treated groups Our results have revealed a significant sex difference in colorectal afferent innervation and sensitization in the context of behavioral visceral hypersensitivity
  • Peripheral and Central P2X3 Receptor Contributions to Colon . . .
    Methods—The visceromotor response (VMR) to colon distension (15 – 60 mmHg) was determined before and after intracolonic saline or zymosan (30 mg mL, 0 1 mL, daily for 3 days) treatment Colon pathology and intracolonic ATP release was assessed in parallel experiments To examine P2X3 receptor contributions to colon mechanosensation and hypersensitivity, electrophysiological experiments





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